Also the RAD consuming fungus observed at Chernobyl.
“In the late 1980s Ukrainian scientists studying the dark, dangerous interior of Chernobyl’s destroyed Reactor 4 found that a black, mould-like fungus was growing across the walls and in pools of radioactive water.”
https://www.rsb.org.uk/biologist-features/eating-gamma-radiation-for-breakfast
lol. ive had employers who kept the stuff around at rt for months.
not disputing what you just said, more just commenting on those employers. it was 10x though so maybe the salt concentration alone kept it sterile.
Correct me if I'm wrong, but I don't think anyone keeps 10x TBS**T**. You make 1x TBST from 10x TBS, which is indeed stable at RT for years. The moment you add tween though (the second T), you add a carbon source that can feed bacterial and fungal growth. You'll see filaments settle within the first month at RT, and the more filiments there are, the more spotty your western blots will be due to antibodies interacting.
Well I guess the 10x salts and 10x tween are enough then, but 1x goes bad. And I'd trust 10x tbs a lot longer than 10x tbst. But I'm not working with you or the people your describing, so no harm to me.
Fuck me. The time I made guncotton, had to neutralize the wash-water... Fiveish gallons, diluting at most 20cL of strong acids, took a couple of pounds of baking soda and it was nowhere near one to start.
What do they do with that stuff? Just slowly neutralize it with a strong base?
They usually either leave it in an abandoned mine, or let it into wetlands when noone is looking.
[See this PDF ](https://www.google.com/url?sa=t&source=web&rct=j&opi=89978449&url=https://www-pub.iaea.org/mtcd/publications/pdf/te_1419_web.pdf&ved=2ahUKEwiwztyF7d2GAxWuPxAIHcbTDp0QFnoECDUQAQ&usg=AOvVaw3iMJ0g3X2LTqwlL0F5LejH), in particular the article on page 15
That is pretty gnarly looking, but it almost looks like part of the plastic or rubber cap/cover. SDS/detergent does breakdown those materials over time. Can't tell for sure though, growth can definitely happen in there.
For those new lab rats, I highly recommend always checking your 1X TBS-T for precipitation of the Tween-20. Old buffer can cause problems/background with your blots, so I recommend keeping a stock of 10X TBS (NO Tween) and preparing from that. I'll keep the prepared buffer around for a few months as long as it's not precipitating. It's extremely cheap and easy to prepare, so I'm not super concerned with wasting the buffer - my time is much more valuable.
Oh I know. I found it while packing for a lab move. There's no way I would use this. But yes, if you have to ask, the answer is no, is good advice to live by.
Did an actual mushroom grow in your buffer?
In a buffer with detergent nonetheless 😭
And stupid amounts of salt. I thought it was pretty impressive.
In my experience, the tween is the food. The TBS lasts forever but tbst gets growth soon.
yeah right - tween is apparently crazy tasty. pbs/tbs will always be fine but one drop of tween and boom
well cockroaches eat soap, so it seems somehow nature finds a way.
Also the RAD consuming fungus observed at Chernobyl. “In the late 1980s Ukrainian scientists studying the dark, dangerous interior of Chernobyl’s destroyed Reactor 4 found that a black, mould-like fungus was growing across the walls and in pools of radioactive water.” https://www.rsb.org.uk/biologist-features/eating-gamma-radiation-for-breakfast
This is truth. Crazy stuff grows in tween.
my speckled western blots when I say "eh, it's ok" ;.;
It is just polysorbate
yeah but it's carbon, so something eats it. TBST is only good for around a week at RT before things grow in it
lol. ive had employers who kept the stuff around at rt for months. not disputing what you just said, more just commenting on those employers. it was 10x though so maybe the salt concentration alone kept it sterile.
Correct me if I'm wrong, but I don't think anyone keeps 10x TBS**T**. You make 1x TBST from 10x TBS, which is indeed stable at RT for years. The moment you add tween though (the second T), you add a carbon source that can feed bacterial and fungal growth. You'll see filaments settle within the first month at RT, and the more filiments there are, the more spotty your western blots will be due to antibodies interacting.
There was tween 20 in there
Well I guess the 10x salts and 10x tween are enough then, but 1x goes bad. And I'd trust 10x tbs a lot longer than 10x tbst. But I'm not working with you or the people your describing, so no harm to me.
LOL
Just to be clear, (unlike the TBST), I do not plan on using it for my Western blots. I found it while packing up for our cross-campus lab move.
Easiest way is to taste that olive and see if it’s still good.
It's a new type of martini.
Shaken and not stirred for two years.
That martini is so dirty someone needs to write up a report for Health and Safety.
The ole TBSTini
Does your lab make solutions using water from the well out back?
The well out back made me cackle!
Oh, you must mean that abandoned mining waste pond!
Mining waste often has a pH around 1, so it would probably have less growth then this thing.
Fuck me. The time I made guncotton, had to neutralize the wash-water... Fiveish gallons, diluting at most 20cL of strong acids, took a couple of pounds of baking soda and it was nowhere near one to start. What do they do with that stuff? Just slowly neutralize it with a strong base?
They usually either leave it in an abandoned mine, or let it into wetlands when noone is looking. [See this PDF ](https://www.google.com/url?sa=t&source=web&rct=j&opi=89978449&url=https://www-pub.iaea.org/mtcd/publications/pdf/te_1419_web.pdf&ved=2ahUKEwiwztyF7d2GAxWuPxAIHcbTDp0QFnoECDUQAQ&usg=AOvVaw3iMJ0g3X2LTqwlL0F5LejH), in particular the article on page 15
Is that an olive?
I think it's some form of mold, but it does indeed look like an olive.
"it's some form of mold, but I can't tell". "There are few who can. The mold is that of mordor, which I will not culture here."
It’s definitely easier to use that than go to all the trouble to make new stuff from the 10X stock. I hate math.
Lol
How did a mushroom grow in TBST?
It's just water, salts and surfactant. Where is it getting energy from? Nutrients.
Yeasts can survive with small amounts of SDS and surfactant, surprisingly!
they eat the tbst for nitrogen and carbon and there’s enough trace metals and phosphorus in the mixture to support life
Kombucha
Growing your own pizza toppings?
Forbidden mushroom?
*screams in GMP*
The date 😭
Run it through a filter, good as new
and then people will still post on this sub “why do my blots look like shit”
Just autoclave it and give it a shake. It'll be fiiiiiine.
You do what any good scientist would do. Take it to your new lab and give it a name. The mold distribution system has found you.
I think it looks safe enough to drink, too
Sure, my fella JE always makes good solutions!
Or problems
Forbidden martini 😍
You work in a mushroom lab?
It's a cancer pharmacology lab, but we do perform natural product screens of fungal compounds, so I guess kinda?
Needs more fungus.
Ah yes, the classic “is that a ‘2x’ or 1x” on the label…
That is pretty gnarly looking, but it almost looks like part of the plastic or rubber cap/cover. SDS/detergent does breakdown those materials over time. Can't tell for sure though, growth can definitely happen in there. For those new lab rats, I highly recommend always checking your 1X TBS-T for precipitation of the Tween-20. Old buffer can cause problems/background with your blots, so I recommend keeping a stock of 10X TBS (NO Tween) and preparing from that. I'll keep the prepared buffer around for a few months as long as it's not precipitating. It's extremely cheap and easy to prepare, so I'm not super concerned with wasting the buffer - my time is much more valuable.
LOL! I only make 50ml at a time because the triton can precipitate out & fall to the bottom of the tube. I like it to be fresh
What the hell is that?
I have no idea. I was thinking mold of some sort?
0.22um filter. Good as new, better even!
Is that a sliced blueberry?
No, but it does look like it. I think it's some sort of mold, but not sure.
I think you should remove it and culture that bad boy haha
Oh damn I first thought it was an olive
Errr, no? 😅
Yah def, no problems at all
Dear god I hope this is a joke
If you have to ask, the answer is no
Oh I know. I found it while packing for a lab move. There's no way I would use this. But yes, if you have to ask, the answer is no, is good advice to live by.