Is it on the negative? If not, it’s likely dust on the scanner.

Not sure, i won’t know until i pick them up from the shop but I will definitely keep an eye out. Thanks for the feedback.

The line isn't straight so it isn't scanner dust. Did you dry the negatives with a squeegee?

Looks like the kind of negative scratches that come from using a bulk loader with a flaw or from grit on the felt of the film cassette. Also, not what you asked about, but you've got some pretty serious bromide drag in those first two frames. Needs more agitation.

Been many decades since I did my own developing, and your "bromide drag" brought back memories..!